The tumor volume measured by magnetic resonance imaging (MRI) and quantitative bioluminescence imaging (BLI) signal showed a correlation coefficient of r = 0.96. Images and behavioral tests were evaluated at base time and at 7, 14, 21, and 28 days after induced tumor growth analysis. The sham group received implant only with culture medium without cells. The experimental group was anesthetized the C6 cells with luciferase expression that were suspended in a culture medium were implanted into the right frontoparietal cortex of the rats. Our sample includes male Wistar rats, 2 months old and weighing 250–300 g, that is categorized into three groups: control, sham, and experimental. The primary objective of this study is to monitor tumor growth by using image techniques and behavioral testing through general and specific motor activities (spontaneous movements and gait). Taylla Klei Felix Souza, Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing, # 1 Mariana Penteado Nucci, Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing, # 2 Javier Bustamante Mamani, Conceptualization, Formal analysis, Investigation, Methodology, Validation, Writing – original draft, Writing – review & editing, 1 Helio Rodrigues da Silva, Conceptualization, Formal analysis, Investigation, Methodology, Visualization, Writing – original draft, Writing – review & editing, 1 Daianne Maciely Carvalho Fantacini, Data curation, Formal analysis, Methodology, Validation, 3 Lucas Eduardo Botelho de Souza, Data curation, Methodology, Visualization, Writing – original draft, Writing – review & editing, 3 Virginia Picanço-Castro, Data curation, Methodology, Writing – original draft, Writing – review & editing, 3 Dimas Tadeu Covas, Data curation, Methodology, Writing – original draft, Writing – review & editing, 3 Edson Luis Vidoto, Data curation, Investigation, Methodology, 4 Alberto Tannús, Data curation, Investigation, Methodology, 4 and Lionel Fernel Gamarra, Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing 1, 5, *

Breaks actimeter